AN11--Degenerative cervical myelopathy (DCM) multi-omics services

This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued. This is a Request for Quote (RFQ) and the solicitation number is 36C24E26Q0072. The government anticipates awarding a firm-fixed price contract resulting from this combined synopsis/solicitation. This solicitation is issued as a 100% set aside for small business concerns. It is the responsibility of the contractor to be familiar with the applicable clauses and provisions. The clauses may be accessed in full text at www.acquisition.gov. The applicable NAICS code is 541380 Testing Laboratories and Services, with a $19.0M employee size standard. The PSC is AN11 Health R & D Services; Health Care Services; Basic Research. **NOTE: RFQ 36C24E26Q0072 hereby replaces RFQ 36C24E26Q0047** STATEMENT OF NEED (SON) CONTRACT DESCRIPTION AND REQUIREMENTS The Regional Procurement Office (RPO) - East has a requirement to purchase multi-omics services for the VA Pittsburgh Healthcare System Research and Development Department in support of VA-funded study. A firm-fixed price contract will be awarded for the purchase of services in accordance with all terms, conditions, provisions, specifications and schedule of this solicitation herein. Quotes shall contain the Offeror s best terms for cost/price and technical capabilities of the requirement. The government reserves the right to award without discussions. 1.1 PRICING The Offeror shall submit price quotes for the required services to the specifications listed in Section 1.2 below. Please enclose the established price list and description of capabilities and product specifications for the requirement using the following format: ITEM INFORMATION ITEM NUMBER DESCRIPTION OF SUPPLIES/SERVICES QUANTITY UNIT UNIT PRICE AMOUNT 0001 40.00 EA _________________ _________________ Transcriptomics (mRNA-seq) Analysis Includes: RNA extraction, Poly-A enrichment, library construction, sequencing (Illumina), and bioinformatics analysis (DEG, GO, KEGG). Sample Type: Whole blood (PAXgene). 0002 40.00 EA _________________ _________________ DIA-Based Quantitative Proteomics Analysis Includes: Protein extraction, Paramagnetic Bead-based preparation, peptide desalination, LC-MS/MS (DIA) data acquisition, and data analysis. Sample Type: Plasma (BD P100 tubes with KEDTA/inhibitors). 0003 40.00 EA _________________ _________________ Untargeted Metabolomics Analysis Includes: Metabolite extraction, LC-MS analysis (Thermo Q Exactive + Waters HSS T3 column), data processing, and statistical analysis. Sample Type: Plasma (Heparin tubes). 0004 40.00 EA _________________ _________________ Multi-Omics Integration Analysis Includes: Integrated pairwise analysis (e.g., Proteome+Metabolome), PLS-DA modeling, and correlation heatmaps. 0005 1.00 EA _________________ _________________ Handling fee (covers sample receiving, inspection, and storage) 0006 1.00 EA _________________ _________________ Setup Fee (one-time; covers initial optimization) 0007 1.00 EA _________________ _________________ Shipping and handling (to include special packaging for shipping samples to vendor and overnight shipping to and from vendor) GRAND TOTAL __________________ Note: The Offeror should include a description of any support services in the technical quote. 1.2 GENERAL INFORMATION Research Support Services - Degenerative cervical myelopathy (DCM) multi-omics Background: Degenerative cervical myelopathy (DCM) is the most common cause of spinal cord dysfunction and poses a significant health burden in Veterans. However, early detection is a significant challenge, leading to the current study aimed at identifying diagnostic biomarkers that will inform more targeted and individualized treatment strategies. This study will employ three differ...